hacat cell line (primary epidermal keratinocytes normal human adult)  (AddexBio Inc)

Journal: Nanomaterials

Article Title: Tattoo Ink Metal Nanoparticles: Assessment of Toxicity In Vitro and with a Novel Human Ex Vivo Model

doi: 10.3390/nano15040270

Figure Lengend Snippet: MTS assay evaluating the toxicity of NP standards in the HaCaT cell line. CdSO 4 was the positive control ( a ). In this cell type, no toxicity was observed with TiO 2 , and ZnO NPs ( b , d ), whereas a dose-dependent toxicity of CuO NPs was observed ( c ). Data are expressed as mean values obtained in different experiments ± standard error (SE). * p ≥ 0.1. ** p ≥ 0.05. *** p ≥ 0.01.

Article Snippet: The human keratinocyte cell line HaCaT (ATCC ® , PCS-200-011) and primary cultures of human keratinocytes and fibroblasts, previously isolated in our institute from donated biopsies from healthy individuals (Ethical Committee protocol n. 581/3, 2019), were used.

Techniques: MTS Assay, Positive Control

Journal: Nanomaterials

Article Title: Tattoo Ink Metal Nanoparticles: Assessment of Toxicity In Vitro and with a Novel Human Ex Vivo Model

doi: 10.3390/nano15040270

Figure Lengend Snippet: MTS assay of toxicity of NP standards in primary human keratinocytes, and of tattoo inks in HaCaT cells. CdSO 4 was the positive control ( a ). In primary human keratinocytes, no toxicity was observed with CuO NPs ( b ), whereas a dose-dependent toxicity was seen only with the ZnO NPs ( c ). The three commercial inks tested in HaCaT cells at different dilutions did not show toxicity in this assay ( d – f ). Data are expressed as mean values obtained in different experiments ± standard error (SE). *** p ≥ 0.01.

Article Snippet: The human keratinocyte cell line HaCaT (ATCC ® , PCS-200-011) and primary cultures of human keratinocytes and fibroblasts, previously isolated in our institute from donated biopsies from healthy individuals (Ethical Committee protocol n. 581/3, 2019), were used.

Techniques: MTS Assay, Positive Control